2720 thermal cycler touchdown pcr full#
2720 thermal cycler touchdown pcr plus#

(Refer to the QuantStudio™ 12K Flex Real-Time PCR System Maintenance and Administration Manual, Appendix C, “Creating a custom dye plate”, for full details. Create a full plate of the dye using the selected concentration and run the custom dye calibration as normal, using 20 µL.Select the concentration to use by finding the dilution that will give you an acceptable signal in the following ranges:įor a 384-well plate: between 400,000 and 1,200,000įor a 96-well plate: between 1,400,000 and 4,300,000 When the run is complete, export and examine the raw data.5-inch intuitive color touchscreen for easy programming and training of new. And because its airflow is front to back, you can set multiple units side by side to save valuable bench space. Ensure that the filters of interest are selected. Key features and benefits: Small footprint With dimensions of 19 cm wide and 39 cm deep, you can fit the MiniAmp Thermal Cycler almost anywhere. With their compact designjust 7.5 inches (19 cm) wideand entry-level price, MiniAmp Thermal Cyclers are perfect for every lab bench.

2720 thermal cycler touchdown pcr plus#

Alter the thermal profile so that it simply ramps to 60☌ with a 2 min hold. Backed by the proven reliability of Applied Biosystems technology, the MiniAmp Plus Thermal Cycler and the MiniAmp Thermal Cycler were designed for routine PCR.

Set up a dummy run using the ‘Standard Curve’ option.

Note: The volume would be the same for a 96-well or 384-well plate. In this example, sample concentrations would be 100, 200, 400, 800, and 1,600 (20 µL/well). Get troubleshooting help for commonly encountered PCR Thermal Cycler errors. Thermocyclers operate by cycling through temperatures instantly, but in practice, temperature changes depend on factors such as the composition of the. A full plate is not needed see the example below. PCR machines, which often accommodate the standard 96-well sample format, are fundamental lab instruments that carry out PCR-based amplification or real-time detection. The following figure shows the emission spectrum for each dye, and the filters and wavelengths at which each dye is read. Use either the background buffer provided in the calibration kit or your own buffer to dilute the oligo. The Applied Biosystems 75 Fast Real-Time PCR Systems use the following dye sets for calibration: C圓, Cy5, FAM, JOE, NED, ROX, SYBR Green, TAMRA, Texas Red, and VIC dyes. For each custom dye, prepare samples in a concentration range of 100–2,000 nM, choosing a 2–3-fold difference in dilution points.

You will need an oligo sequence with the custom dye but without the quencher molecule. PC-controlled or run directly from touch screen Gene expression, genotyping, copy number variation, HRM, protein thermal shift, protein detection, mutation detection, miRNA, presence/absence, digital PCR Thermal Cycler for use with applications licenses available from Applied Biosystems. 96-well (standard), 96-well (Fast), 384-well, TaqMan® Array Card, OpenArray® plate (4 plates)įAM™, SYBR®, VIC®, ROX™, NED™, TAMRA™ dyesġ0–100 µL (96-well standard) 15–30 µL (96-well Fast) 5–20 µL (384-well) ~1 µL (TaqMan® Array Card) 33 nL (OpenArray®Plate)Ħ excitation filters (450–670 nm), OptiFlex™ system (enhanced fluorescence detection), 6 emission filters (500–720 nm)įor Research Use Only.